ST1122
Anti-Rictor Mouse mAb (2C8)
Anti-Rapamycin-Insensitive Companion of mTOR Mouse mAb
Para informações, por favor, contate o nosso Serviço de Atendimento ao Cliente
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefone: +49 6151 72-0
Fax: +49 6151 72 2000
13 Fevereiro 2012
carregando
| Nº de produto | Tamanho |
|---|---|
| ST1122-50UG | 50 μg |
Recognizes the ~190 kDa Rictor protein in HEK293 cells.
| Informações sobre o produto | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG2a | ||
| Immunogen | a recombinant protein consisting of amino acids 1-99 of human Rictor fused to GST | ||
| Clone | 2C8 | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Sim | ||
| Positive control | HEK293 cells | ||
| Informação de estoque e transporte | |||
|---|---|---|---|
| Armazenagem | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Dados | |||
|---|---|---|---|
 Lane 1: Detection of human Rictor by immunoblotting. Sample: Whole cell lysate (40 µg) from HEK293 cells. Primary antibody: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (1 µg/ml). Detection: chemiluminescence. Lanes 2 and 3: Detection of mTOR/Rictor by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Antibody for IP: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (10 µg/mg total protein). Immunoblotting conditions: Samples: half of Rictor IP per lane. Primary antibodies: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (1 µg/ml) (Lane 2) and Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) (Cat. No. OP97) (1 µg/ml) (Lane 3). Detection: chemiluminescence. |
